As noted in the OP the 'round of 64' 1st round has only been around since the women's tournament expanded from 48 to 64 in 1994, thus "30 years" and '30-0' (the reason its not 31 years/31-0 is the suspended COVID year of 2020).
Auriemma's teams have, however, lost twice in the prior 'round of 32' 1st round (1989, 1993), as well as their 1st game in the 1990 tournament when they received a 1st round bye to the 2nd round.