I'd probably get outvoted on this, but Washington was the best one to me. They were all great, but I was too new a fan in 1990 to fully appreciate the Tate shot, Florida was regular season and against Pitt the game was tied. Washington had everything -- we went from losing to winning, in the NCAA tournament, and plus there was the pandemonium with that ball bouncing all over the place. I was sure the buzzer was going to go off any second, and then Rip gets it in his hands and we win.
Also, this video is outstanding.